AI-based antibody humanization service
The CDR regions of antibodies are mainly involved in the interaction between antibodies and antigens. The most common humanization approach is to transplant these CDR regions that determine antigen-antibody interactions into the framework (FR) regions of humans, thereby reducing the immunogenicity of murine antibodies in the human body. The method of CDR transplantation was first proposed by the team of Greg Winter, the Nobel Prize in Chemistry laureate in 2018, in the late 1980s. After decades of development, this technology is currently relatively mature and is the most widely used and recognized humanization strategy in the industry.
Humanization strategy as follows:
1. Extract variable region sequences and predict variable region structures using AI models.
2. Comparison of human templates and CDR transplantation: Select the optimal template combination based on homology, druggability, literature data, pI, etc.
3. Score key residues that affect antibody conformation, function, and stability, restore mutations, and generate humanized variant sequences. Generate patent examples, predict PTM risks, and predict immunogenicity risks.
4. Express and identify candidate humanized variants.
Advantages as follows:
